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Antibody responses to individual antigens in related to M. avium subsp. paratuberculosis shedding levels. Bars represent the means OD values at 405 nm. Error bars represent standard deviations from the means. All recombinant antigens showed increases of antibody responses according to shedding levels and antibody responses to the 35-kDa protein were positively separated between noninfected healthy cows and both shedders (P < 0.01).
