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. 2005 Aug;73(8):5074–5085. doi: 10.1128/IAI.73.8.5074-5085.2005

FIG. 4.

FIG. 4.

FIG. 4.

Changes in T-cell subset distribution in bovine peripheral blood lymphocytes after stimulation with recombinant antigens as determined by FACS analysis. (A) CD4. Ag 85A and Ag 85B induced a higher proportion of CD4+ T lymphocytes in medium shedders than in low shedders, while the percentage of CD4+ lymphocytes was unchanged in noninfected control cattle. (B) CD8. Ag 85A increased the proportion of CD8+ T lymphocytes in medium shedders, while the increased percentage of CD8+ lymphocytes was very low in noninfected cattle. (C) CD25. Ag 85A and Ag85B increased the proportion of CD25+ T cells in both shedder groups, while they had little effect in noninfected cattle. In contrast, Ag 85C and the 35-kDa protein significantly increased the proportion of CD25+ T cells in only the medium shedders (P < 0.05). (D) γδ+ T cells. All antigens resulted in significantly lower increases in all cell subsets in both the low and medium shedder groups except SOD for γδ+ T cells in medium shedders.