FIG. 6.

Increased CD21⁺ B lymphocyte subsets in bovine peripheral blood lymphocytes after stimulation with recombinant antigens as determined by FACS analysis. The results are reported as the average percent increase in positive-staining cells, and the error bars represent 1 standard error of the mean. Recombinant 35-kDa protein induced the largest increase in CD21⁺ B lymphocytes in medium shedders. No significant increase in the proportion of B lymphocytes was observed in response to the other antigens regardless of bacterial shedding levels (P > 0.05).