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Comparison of cytokine mRNA profiles for IFN-γ (A), IL-12p40 (B), and TNF-α (C) of bovine PBMCs from noninfected cattle and low and medium shedders after stimulation with recombinant antigens for 24 h. Results represent the mean fold increase over unstimulated PBMCs, which served as calibrators. The results are similar with Ags 85A and 85B most strongly stimulating medium shedders, while the 35-kDa protein and SOD had lesser effects.
