TABLE 1.
Sequences of PCR primers and TaqMan probes specific for bovine GAPDH and cytokines
| Cytokine and primers | Sequence (5′-3′) | Length | Probe | Probe sequence (5′-3′) | Accession no. |
|---|---|---|---|---|---|
| GAPDH | 67 | P-492 | CACTGTCCACGCCATCACTGCCA | AF077815 | |
| F-470 | GCATCGTGGAGGGACTTATGA | ||||
| R-536 | GGGCCATCCACAGTCTTCTG | ||||
| IL-12p40 | 101 | P-906 | TGCCAACGTCCGCGTGCAA | U11815 | |
| F-884 | CCACCGTCACATGCCACAAGG | ||||
| R-944 | CTGTAGTAGCGGTCCCGGG | ||||
| IL-4 | 66 | P-332 | TCCTGGGCGGACTTGACAGGAATC | M77120 | |
| F-311 | GCCACACGTGCTTGAACAAA | ||||
| R-376 | TCTTGCTTGCCAAGCTGTTG | ||||
| IL-2 | 81 | P-317 | CCCCAGAGATCAAGGATTCAATGGACA | M12791 | |
| F-293 | TTTAGCTCCAAGCAAAAACCTGA | ||||
| R-373 | TGTAGTTCCAAAACGATTCTCTTCA | ||||
| IFN-γ | 106 | P-564 | TCTCTTTCGAGGCCGGAGAGCATCA | M29867 | |
| F-504 | CAGAAAGCGGAAGAGAAGTCAGA | ||||
| R-609 | CAGGCAGGAGGACCATTACG | ||||
| TNF-α | 118 | P-3310 | AACCAGCCTCAGAGTTTCAGCGAGTTCC | Z14137 | |
| F-3261 | TCTCCGTGCAAAAGTTGGG | ||||
| R-3378 | CGAACTCCAGCACAGCAGG |