FIG. 2.

Demonstration of surface expression of bovine CD18 chimeric antigens in various transductants. Surface expression of chimeric CD18 antigens in the various transductants was confirmed by FACS using anti-CD18-specific MAb BAQ30A. The parent cell line K562 cells (open trace) which do not express CD18 antigen were used as a negative control. The x axis shows fluorescence intensity (FL2-H), the y axis shows cell numbers (counts), and the numbers shown within the panels are the percentages of positive CD18 cells. Results show high levels of expression of CD18 antigen in all chimeric transductants (filled traces). Data presented are representative of one of three experiments performed. FL2-H, height for phycoerythrin; M1, marker 1.