FIG. 3.

Western blot demonstrating binding of LktA to immobilized chimeric CD18 from various transductants. Cell lysates from various transductants were processed as described in the text. The different chimeric CD18s were immunoprecipitated from cell lysates with MAb BAQ30A coupled to Sepharose CL-4B beads and incubated separately with LktA, followed by detection of bound LktA with an anti-LktA MAb. LktA binds to chimeric transductants B600H, B700H, H110B, H200B, H400B, and H500B but not to B110H, B200H, B400H, and H600B. Bovine CD18 immunoprecipitated from BoLFA-1 cell lysate and human CD18 immunoprecipitated from KL/4 cell lysate served as controls. Data shown are from one representative experiment of three experiments performed.