FIG. 5.

LktA-induced cytolysis in the chimeric transductants. Cytolysis of various cell lines exposed to 100 units of LktA (100 LU/ml) was measured by the XTT assay as described in the text. Transductants B600H, B700H, H110B, H200B, H400B, and H500B showed marked cytotoxicity compared to that obtained with the parent cell line. By contrast, transductants B110H, B200H, B400H, and H600B showed lower cytotoxicity upon exposure to LktA, comparable to that obtained with parent cell line. The BoLFA-1 (BoCD11a/BoCD18), KL/4 line (HuLFA-1, HuCD11a/HuCD18), and parent K562 cell transductants served as controls. Results are expressed as means ± standard errors of the means for three separate experiments. Values that are significantly different from the negative control value (P < 0.05) are indicated by asterisks.