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. 2005 Aug;73(8):4810–4817. doi: 10.1128/IAI.73.8.4810-4817.2005

FIG. 1.

FIG. 1.

Schematic drawing of plasmid pML1 and construction of plasmid pSNUCIQ3. Plasmid pML1, encoding the lysis protein E, and plasmid pSNUCIQ3, encoding SNUC, were used for the coexpression of gene E and SNUC in E. coli O157:H7. The expression of the killing genes was triggered either by the addition of a chemical inducer (pSNUCIQ3) or by a thermal shift from 28°C to 42°C (pML1). E, lysis gene E; Amp, ampicillin resistance gene; Kan, kanamycin resistance gene; Tet, tetracycline resistance gene; ColE1 and p15A, origins of replication; A1-O4/O3, synthetic, chemically inducible promoter; PRMand PR, rightward “maintenance” and rightward promoters of bacteriophage lambda, respectively; cI857, gene encoding the thermosensitive repressor for the lambda PR promoter; lacIq, gene encoding the repressor for the synthetic A1-O4/O3 promoter; XhoI, PstI, and SalI, restriction sites used for the construction of pSNUCIQ3.