FIG. 3.
DNA amplification by the Salmonella 5′ nuclease PCR assay in the presence of 5 μl of BHI and 5 μl of BPW. The graphs show the results of the CT response at a constant Salmonella DNA concentration (2 × 10−9 g/microwell) while the concentrations of BHI and BPW in the media were changed. The experiment was performed with both the AmpliTaq Gold mixture and the rTth mixture. The PCR results correspond to the following DNA amplification combinations: AmpliTaq Gold mixture and BPW medium (⧫), AmpliTaq Gold mixture and BHI medium (▴), rTth mixture and BPW medium (⋄), and rTth mixture and BHI medium (▵).