TABLE 1.
Oligonucleotides used for construction of LcrV variants
| Plasmid | Deletion | Sequence of forward primer | Sequence of reverse primer |
|---|---|---|---|
| pEM1 | Δ1-30 | AACATATGGTTTTAGAAGAATTGGTTCAGTT | AAGGATCCTTTACCAGACGTGTCATC |
| pEM2 | Δ31-60 | AAGGTACCAATAGAGTAATTACTGATGATATC | AAGGATCCTTTACCAGACGTGTCATC |
| pEM3 | Δ61-90 | AACATATGATTAGAGCCTACGAACAA | AAGGTACCGGCAAAAACCTCCGAATCTTT |
| pEM3 | Δ61-90 | AAGGTACCGACAACCAACTGCAAAATGG | AAGGATCCTTTACCAGACGTGTCATC |
| pEM4 | Δ91-120 | AACATATGATTAGAGCCTACGAACAA | AAGGTACCATAATGACCGCCTTTAAGAATG |
| pEM4 | Δ91-120 | AAGGTACCGTAATGCATTTCTCTTTAACCG | AAGGATCCTTTACCAGACGTGTCATC |
| pEM5 | Δ121-150 | AACATATGATTAGAGCCTACGAACAA | AAGGTACCTGCCATGAACGCCCGCAAT |
| pEM5 | Δ121-150 | AAGGTACCAGCAAGTTGCGTGAAGAATTA | AAGGATCCTTTACCAGACGTGTCATC |
| pEM6 | Δ151-180 | AACATATGATTAGAGCCTACGAACAA | AAGGTACCACGGGCATCACCATGATGAT |
| pEM6 | Δ151-180 | AAGGTACCAGTGGCACCATAAATATCCAT | AAGGATCCTTTACCAGACGTGTCATC |
| pEM7 | Δ181-210 | AACATATGATTAGAGCCTACGAACAA | AAGGTACCACTAGACAGATGCTTATTAATTT |
| pEM7 | Δ181-210 | AAGGTACCGCAGAGTACAAAATTCTCGAG | AAGGATCCTTTACCAGACGTGTCATC |
| pEM8 | Δ211-240 | AACATATGATTAGAGCCTACGAACAA | AAGGTACCGCTGGCTTTAAAAATCTCTTCA |
| pEM8 | Δ211-240 | AAGGTACCGGAAGTGAGAATAAAAGAACC | AAGGATCCTTTACCAGACGTGTCATC |
| pEM9 | Δ241-270 | AACATATGATTAGAGCCTACGAACAA | AAGGTACCAAGAAAGTCCTTTATCGAGACT |
| pEM9 | Δ241-270 | AAGGTACCACCACCTGCTCGGATAAGT | AAGGATCCTTTACCAGACGTGTCATC |
| pEM10 | Δ271-300 | AACATATGATTAGAGCCTACGAACAA | AAGGTACCGGCAAAGTGAGATAATTCATTAT |
| pEM11 | Δ301-326 | AACATATGATTAGAGCCTACGAACAA | AAGGATCCTGAATTAAAACGTGATGTAATATC |