FIG. 2.

Images of 28SC cells infected with C. jejuni 81-176. (A and B) Cells infected with 81-176 carrying pCPE111/28/GFP under phase (A) or fluorescence (B). The GFP gene from plasmid pWM1007 (16) was PCR amplified and cloned into plasmid pCPE111/28, a kanamycin resistance shuttle plasmid (23). This expression vector is similar to the chloramphenicol-resistant vector described by Larsen et al. (14). (C and D) Cells infected with wild-type 81-176 and stained with BacLight live/dead stain. The monocytes in panels C and D are dead but contain live C. jejuni organisms in vacuoles. 28SC cells were inoculated as described in the legend to Fig. 1.