TABLE 1.
Cell lines used for this study
| Cell line | Reference or sourcea (no.) | Cell type | Growth mediumb |
|---|---|---|---|
| 293T | ATCC (CRL 11268) | Human embryonal kidney | DMEM (Gibco BRL), 10% fetal bovine serum (FBS; Gibco BRL), 2 mM l-glutamine, 1 mM sodium pyruvate |
| AGS | ATCC (CRL 1739) | Human gastric adenocarcinoma | RPMI 1640 (Gibco BRL), 10% FBS, 2 mM l-glutamine |
| CHO K1 | ATCC (CCL 61) | Chinese hamster ovary | MEM alpha (Gibco BRL), 10% FBS |
| Cos1 | ATCC (CRL 1650) | Green African monkey kidney | RPMI 1640 (Gibco BRL), 10% FBS, 2 mM l-glutamine |
| GLC4 | 42 | Human lung carcinoma | RPMI 1640 (Gibco BRL), 10% heat-inactivated FBS, 2 mM l-glutamine |
| Hec1.b | ATCC (HTP 113) | Human endometrial adenocarcinoma | RPMI 1640 (Gibco BRL), 10% FBS, 2 mM l-glutamine |
| Hela | ATCC (CCL 2) | Human cervix epithelial carcinoma | RPMI 1640 (Gibco BRL), 10% FBS, 2 mM l-glutamine |
| HepG2 | ATCC (HB 8065) | Human hepatocellular carcinoma | RPMI 1640 (Gibco BRL), 10% FBS, 2 mM l-glutamine |
| HL | ECACC (96121720) | Human lung, fibroblast-like | RPMI 1640 (Gibco BRL), 10% FBS, 2 mM l-glutamine |
| HT29 | ATCC (HTB 38) | Human colon adenocarcinoma | Mc Coy's 5A (Gibco BRL), 10% FBS |
| J774.A | DSMZ (ACC 170) | Mouse monocytic macrophages | RPMI 1640 (Gibco BRL), 10% heat-inactivated FBS, 2 mM l-glutamine |
| Kato3 | ATCC (HTB 103) | Human gastic carcinoma | RPMI 1640 (Gibco BRL), 10% heat-inactivated FBS, 2 mM l-glutamine |
| L929 | ATCC (CCL 1) | Mouse fibroblasts | RPMI 1640 (Gibco BRL), 10% FBS, 2 mM l-glutamine |
| MDCK | ATCC (CCL 34) | Canine kidney | RPMI 1640 (Gibco BRL), 10% FBS, 2 mM l-glutamine |
| MKN-28 | 27 | Human gastric adenocarcinoma | RPMI 1640 (Gibco BRL), 10% heat-inactivated FBS, 2 mM l-glutamine |
| MKN-45 | DSMZ (ACC 409) | Human gastric adenocarcinoma | RPMI 1640 (Gibco BRL), 10% heat-inactivated FBS, 2 mM l-glutamine |
| SR4987 | DSMZ (ACC 323) | Mouse stromal fibroblast-like | Mc Coy's 5A (Gibco BRL), 10% FBS |
| SYF+src | ATCC (CRL 2498) | Mouse fibroblasts overexpressing Src | DMEM (Gibco BRL), 10% FBS, 2 mM l-glutamine, 1 mM sodium pyruvate |
| THP1 | ATCC (TIB 202) | Human monocytes from acute monocytic leukemia | RPMI 1640 (Gibco BRL), 10% heat-inactivated FBS, 2 mM l-glutamine |
a
ATCC, American Type Culture Collection (www.atcc.org); DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (www.dsmz.de); ECACC, European Collection of Cell Cultures (www.ecacc.org.uk).
b
DMEM, Dulbecco's modified Eagle's medium; MEM, minimum essential medium.