TABLE 2.
Responses of 19 mammalian cell lines to infection with H. pylori strain P1
| Cell type | Cell line | Organism | Tissue | CagA phosphory- lationa | CagA pro- cessing | Host cell protein dephosphory- lation | Cellular phenotype | IL-8 secretionb |
|---|---|---|---|---|---|---|---|---|
| Human gastric cells | AGS | Human | Stomach | +++ | − | + | Hummingbird (CagA dependent) | + |
| MKN-45 | Human | Stomach | +++ | + | + | + | ||
| MKN-28 | Human | Stomach | ++ | − | − | + | ||
| Kato 3 | Human | Stomach | +++ | − | + | + | ||
| Human nongastric cells | HT29 | Human | Colon | ++ | − | + | + | |
| HeLa | Human | Cervix | ++ | − | − | + | ||
| GLC4 | Human | Lung | − | − | − | − | ||
| Hec1.b | Human | Endometrium | + | − | + | + | ||
| 293T | Human | Kidney | ++ | − | + | + | ||
| HL | Human | Lung | ++ | − | − | + | ||
| THP1 | Human | Blood | + | + | − | Homotypic aggregation (TFSS | + | |
| HepG2 | Human | Liver | ++ | − | + | dependent) | + | |
| Nonhuman nongastric | CHO K1 | Hamster | Ovary | − | − | − | ND | |
| cells | Cos-1 | African green monkey | Kidney | + | − | − | ND | |
| J774.A | Mouse | Blood | ++ | + | − | +(Mip-2) | ||
| MDCK | Dog | Kidney | − | − | − | ND | ||
| SR 4987 | Mouse | Bone marrow | + | − | − | − (Mip-2) | ||
| SYF+src | Mouse | Connective tissue | + | − | − | − (Mip-2) | ||
| L929 | Mouse | Connective tissue | + | − | − | − (Mip-2) |
a
Intensity of CagA-tyrosine phosphorylation relative to that in AGS cells (see Fig. 1A for further details).
b
At least a twofold increase in IL-8 secretion compared to that by P1ΔvirB11 is indicated by “+”(see Fig. 2). With the mouse cell lines, we analyzed the secretion of the murine IL-8 analog Mip-2, as indicated. Proinflammatory responses in CHO K1, Cos-1, and MDCK cells could not be determined (ND). For details, see Materials and Methods.