FIG. 5.

In vitro antibacterial activities of different HD5 forms with various N termini. Dose response relationships with recombinant proHD5 (aa 20 to 94), HD5 (aa 56 to 94), and HD5 (aa 63 to 94), tested at the same molarities, are shown. (A) For antigonococcal activity, defensins were mixed with 24-hour cultures of N. gonorrhoeae adjusted to ∼2 × 10⁶ CFU/ml in 70% RPMI supplemented with glucose and IsoVitaleX, and the number of CFU were determined after a 3-hour incubation. Values are expressed as change over the control at time zero. (B) For anti-C. trachomatis activity, defensins were added to infectious EB in SPG medium for 45 min prior to infection of Ishikawa cells. The inclusion-forming units (IFU)/well were enumerated 40 h after infection by immunohistochemistry using an anti-chlamydia lipopolysaccharide antibody. Data are expressed relative to the IFU/well resulting from infection with untreated chlamydia. Depicted are the means + standard deviations of three experiments.