Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Sep 9;33(16):5112–5119. doi: 10.1093/nar/gki824

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2005. Published by Oxford University Press. All rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

PMC Copyright notice

In vitro splicing efficiency of pre-mRNAs with hyper- and hypostabilized interaction with U1 snRNA. Representative reaction profiles are shown for pre-mRNAs with an extended U1 snRNA interaction potential (A) and a pre-mRNA with a low U1 snRNA interaction potential (B). Each time course was used to determine observed rates of splicing at each temperature tested. The average rate of splicing is shown below each time course. Rate determinations were repeated at least three times. The nucleotide sequence above each autoradiogram represents the 5′ splice site sequences of the test pre-mRNA. Capital letters symbolize base pairing with U1 snRNA; small letters symbolize mismatched nucleotides with U1 snRNA. The vertical line (|) indicates the intron/exon junction. The identities of spliced and unspliced RNAs are indicated on the right.