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. 2005 Aug 11;24(17):2989–2999. doi: 10.1038/sj.emboj.7600764

Figure 6.

Figure 6

Overexpression of OSH7CC causes an MVB sorting defect. WT cells (Y10000) expressing Osh7CC/Osh7p from pESCOSH7CC/pESCOSH7 were grown in glucose medium to mid-log phase and transferred to galactose/raffinose medium for 4 h. (A) GFP-CPS was visualized using a Leica fluorescence microscopy via a GFP filter. DIC: differential interference contrast. Scale bar: 5 μm. (B) Western blot analysis of total cell extracts from indicated strains with anti-GFP antisera. Equivalent amounts of total protein were loaded in each lane.