FIG. 2.

Detection of C. trachomatis by RAM assay. (A) The RAM reactions were initiated with the synthetic C. trachomatis targets from 10⁷ to 10 molecules as described in Materials and Methods. The RAM products were examined on a 2% agarose gel after digestion with EcoRI. The results show that 10 molecules of C. trachomatis targets were detected. (B) The sensitivity was further confirmed with C. trachomatis EB that were diluted to 250, 100, and 10 copies. As few as 10 copies of C. trachomatis EB can be detected. Lane 0, no target present. (C) RAM products amplified from 10 patient-derived specimens (no. 1 through no. 10) were examined through agarose gel electrophoresis after EcoRI digestion. The presence of a 124-bp band indicates positivity for C. trachomatis.