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Localization of Pmo25 in SIN mutants. (A) pmo25 mutants having cdc7+:GFP:kanr grown in YES medium at 25°C were shifted to 36°C, incubated for 4 h, and fixed. (B, C) SIN mutants having pmo25+:GFP:kanr grown at 25°C were shifted to 36°C, incubated for 4 h, and fixed (B). A summary of the Pmo25 localization is shown in panel C. w, weaker than in WT. (D) The cps1 mutants having pmo25+:GFP:kanr grown at 25°C were shifted to 36°C, incubated for 4 h, and fixed.
