Figure 3.

Effect of divalent metal ion on the fidelity and lesion bypass ability of Dpo4. (A) Specificity of nucleotide incorporation. (B) Mispaired primer extension. Bypass of (C) a synthetic abasic site and (D) a cis-syn CPD. (E) Terminal nucleotidyl transferase activity of Dpo4. Reactions were performed in the presence of 5 mM Mg²⁺ or Mn²⁺. The local template sequence is shown to the left of each gel. Dpo4 concentration in each reaction was 2 nM, or as indicated at the bottom of the gels. Primer elongations were calculated as the percent of total primer termini, and are indicated at the bottom of the gels.