Skip to main content
. 2005 Aug 18;24(17):2957–2967. doi: 10.1038/sj.emboj.7600786

Figure 5.

Figure 5

Comparison of extension of a preformed primer/template terminal mismatch and mismatch formed as a result of nucleotide misincorporation. (A) PPi-dependent inhibition of primer extension in the presence of 100 μM incorrect dGTP and 10 μM of the next correct dCTP. (B) PPi-dependent inhibition of G:T mismatch extension in the presence of 10 μM correct dCTP and dGTP. Reactions were performed in the presence of 8 nM Dpo4. The local template sequence is shown to the left of the gels. Mismatch extension was calculated as 100 × (N1+N2+⋯+Nn)/(Nm+N1+N2+⋯+Nn). Primer elongation was calculated as 100 × (Nm+N1+N2+⋯+Nn)/(N0+Nm+N1+N2+⋯+Nn).