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. 2005 Sep 7;24(17):3158. doi: 10.1038/sj.emboj.7600806

Figure 9.

Figure 9

VE-PTP, but not VE-PTP-R/A, reduces paracellular permeability in VE-cadherin/Flk-1/VE-PTP triple-transfected CHO and inhibits tyrosine phosphorylation of VE-cadherin. Paracellular permeability for FITC-dextran was analysed with monolayers of various stably transfected CHO cells. Left graph: Analysis of triple-transfected CHO-F12, transfected with VE-cadherin, VEGFR-2 and mifepristone-inducible FLAG-VE-PTP. Stimulation with mifepristone for 10 h resulted in a 4574% reduction of cell permeability (black) as compared to mockstimulated cells (light grey). Right graph: Analysis of triple-transfected CHO-RA25.5, transfected with VE-cadherin, VEGFR-2 and mifepristoneinducible FLAG-VE-PTP-R/A. Stimulation with mifepristone for 10 h resulted in no significant reduction of cell permeability (black) as compared to mock-stimulated cells (light grey). Upper panel: CHO-F12 and CHO-RA25.5 cells, either induced (+) or not induced (−) with mifepristone were analysed by immunoprecipitations with anti VE-cadherin antibodies, followed by immunoblotting with an antibody against phospho-tyrosine (anti-PY). The same filter was reprobed with an antibody against VE-cadherin, demonstrating equal loading of VE-cadherin.