Figure 2.

Construction of cell lines expressing fusion proteins targeted to the mitochondrial matrix. (A) Structure of two chimeric gene expression vectors. The mitochondrial leader sequence from the mouse mitochondrial transcription factor A (TFAML) was cloned at the 5′ end of the each structural gene in frame using the pcDNA6/HisA vector. (B) Fluorescence microscopy of HeLa 229 cells expressing GFP targeted to the mitochondrial matrix. The HeLa 229 cells were transfected with the TFAML-GFP chimeric gene expression vector and subjected to fluorescence microscopy after 24 h incubation at 37°C in a 5% humidified CO₂ incubator.