. 2005 Sep 12;33(16):e139. doi: 10.1093/nar/gni140

Table 1.

Various mating and transcription buffer conditions for E.coli growth and in organello RNA synthesis

Incubation buffers	1	2	3	4	5	6	7	8	9	10	11
TB	+	+	+								+
M9 Salts	+	+		+	+	+
PBS							+	+	+
0.5% YE			+			+			+
1% Glucose	+	+	+	+	+	+	+	+	+
1 mg/ml Thiamin proline	+	+		+	+		+	+
20 Amino acids mixture	+	+		+	+		+	+
1 mM ATP	+	+		+	+		+	+			+
1 mM ADP		+			+			+
1 mM Pyruvate	+	+		+	+		+	+			+
1 mM Succinate		+			+			+
LB										+
E.coli growth (OD₆₀₀)	0.6	0.7	1.2	0.2	0.3	0.9	0.6	0.7	1.3	1.8	0

TB, transcription buffer (10% glycerol, 40 mM Tris–HCl, pH 7.4, 25 mM NaCl, 5 mM MgCl₂ and 1 mg/ml BSA); M9 salts, 12.8 g of Na₂HPO₄·7H₂O, 3.0 g of KH₂PO₄, 0.5 g of NaCl and 1.0 g of NH₄Cl in 1 liter of deionized H₂O (22); PBS, phosphate-buffered saline; YE, yeast extracts; LB, Luria–Bertani (10 g of bacto-trypton, 5 g of bacto-yeast extracts, 5 g of NaCl in 1 liter of deionized H₂O, pH 7.4).