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. 2002 Jan;40(1):61–67. doi: 10.1128/JCM.40.1.61-67.2002

TABLE 1.

Locations of PCR and protein fragments within the HCV genome and sequences of primers

Fragment no. Location of:
Primers
PCR fragment (nt) Protein fragment (aa)
27 6246–6530 1973–2067 5′-TCCGGCTCGTGGCTAAAGGAT,
5′-CGTGGTGTATGCGTTGATGGCCTT
28 6345–6647 2006–2106 5′-CTCCCTTTCCTGTCATGCCAA,
5′-GGTCATGCCCGTCACGTAGTGG
29 6423–6764 2032–2145 5′-GGAGCACAGATCACCGGACAT,
5′-GACCTCCTCTCGTAGGAGAGG
30 6507–6812 2060–2161 5′-TTCCCCATCAACGCATACACC,
5′-TGGGAGCTGTGACCCGACCA
31 6582–6899 2085–2190 5′-GTGGCTGCTGAGGAGTACGTGGA,
5′-CAGCCTACGCTTGGCCGTCTC
32 6705–7016 2126–2229 5′-GATGGAGTACGGTTGCACAGG,
5′-GGCCTCGATGAGGTCAGCGTCC
33 6798–7109 2157–2260 5′-GGGTCACAGCTCCCATGTGAG,
5′-CCGAATCGGATCGAAAGAGTCC
34 6876–7199 2183–2290 5′-GCAGAGACGGCCAAGCGTAGGCT,
5′-GCGTGCCCATATGGGCAACGCT
35 6963–7268 2212–2313 5′-AAGGCGACATGTACTACCCATC,
5′-CCCGTGTACCACCGGGGGGACGT
36 7047–7349 2240–2340 5′-AACATCACCCGTGTGGAGTCAGA,
5′-CACGGTGGACTCTGTCAGGAC
37 7140–7445 2271–2372 5′-CCGGCGGAGATCCTGCGAAAAC,
5′-GGCCTGATCGGGAGGGCCAGT
38 7197–7490 2290–2387 5′-CGCCCGGATTACAACCCTCC,
5′-GGAGGAGTACGACTCAACGTC
39 7287–7586 2320–2419 5′-AAGGCCCCCCCAATACCACCTC,
5′-GCAGCAGACGACGTCCTCACC
40 7377–7685 2350–2452 5′-AAGACCTTTGGCAGCTCCGGGT,
5′-GTGACGCAGCAAAGAGTTGCT
41 7470–7769 2381–2480 5′-GACGTTGAGTCGTACTCCTCCA,
5′-GTCCAGGACTTGCAGTCTGTC
42 7566–7898 2413–2523 5′-GGTGAGGACGTCGTCTGCTGC,
5′-CCCGTAGCCAAATTTGGATTT
43 7632–7934 2435–2535 5′-GCGGAGGAGAGCAAGTTGCCCA,
5′-GACGGCCCTGCTGGATAGGCT