Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | Vas | FlyBase | FBgn0283442 | |
| Gene (D. melanogaster) | Spn-E | FlyBase | FBgn0003483 | |
| Gene (D .melanogaster) | Tej | FlyBase | FBgn0033921 | |
| Gene (D. melanogaster) | Tapas | FlyBase | FBgn0027529 | |
| Gene (D. melanogaster) | Qin | FlyBase | FBgn0263974 | |
| Gene (D. melanogaster) | Kots | FlyBase | FBgn0038191 | |
| Gene (D. melanogaster) | Krimp | FlyBase | FBgn0034098 | |
| Gene (D. melanogaster) | Squ | FlyBase | FBgn0267347 | |
| Gene (D. melanogaster) | Mael | FlyBase | FBgn0016034 | |
| Gene (D. melanogaster) | Aub | FlyBase | FBgn0000146 | |
| Gene (D. melanogaster) | AGO3 | FlyBase | FBgn0250816 | |
| Gene (D. melanogaster) | Papi | FlyBase | FBgn0031401 | |
| Gene (D. melanogaster) | Vret | FlyBase | FBgn0263143 | |
| Gene (D. melanogaster) | Bel | FlyBase | FBgn0263231 | |
| Gene (D. melanogaster) | Zuc | FlyBase | FBgn0261266 | |
| Gene (D. melanogaster) | Cup | FlyBase | FBgn0000392 | |
| Gene (D. melanogaster) | Tral | FlyBase | FBgn0041775 | |
| Gene (D. melanogaster) | Me31B | FlyBase | FBgn0004419 | |
| Gene (D. melanogaster) | Shu | FlyBase | FBgn0003401 | |
| Gene (D. melanogaster) | BoYb | FlyBase | FBgn0037205 | |
| Gene (D. melanogaster) | Piwi | FlyBase | FBgn0004872 | |
| Gene (D. melanogaster) | Mei-W68 | FlyBase | FBgn0002716 | |
| Gene (D. melanogaster) | CSN3 | FlyBase | FBgn0027055 | |
| Gene (D. melanogaster) | Pka-C1 | FlyBase | FBgn0000273 | |
| Gene (D. melanogaster) | Twf | FlyBase | FBgn0038206 | |
| Gene (D. melanogaster) | Brn | FlyBase | FBgn0000221 | |
| Gene (D. melanogaster) | Vps25 | FlyBase | FBgn0022027 | |
| Gene (D. melanogaster) | Nup44A | FlyBase | FBgn0033247 | |
| Gene (D. melanogaster) | Nclb | FlyBase | FBgn0263510 | |
| Gene (D. melanogaster) | Spn-D | FlyBase | FBgn0003482 | |
| Gene (D. melanogaster) | Jagn | FlyBase | FBgn0037374 | |
| Gene (D. melanogaster) | Rab7 | FlyBase | FBgn0015795 | |
| Gene (D. melanogaster) | Baf | FlyBase | FBgn0031977 | |
| Gene (D. melanogaster) | Mats | FlyBase | FBgn0038965 | |
| Gene (D. melanogaster) | Abo | FlyBase | FBgn0000018 | |
| Gene (D. melanogaster) | Rab11 | FlyBase | FBgn0015790 | |
| Gene (D. melanogaster) | Vls | FlyBase | FBgn0003978 | |
| Gene (D. melanogaster) | Hsc70-4 | FlyBase | FBgn0266599 | |
| Strain, strain background (Escherichia coli) | DH5α | Takara | Cat# 9057 | Competent cells |
| Genetic reagent (D. melanogaster) | w-; squHE47 cn bw/CyO; TM3 Sb/TM6 Tb | Pane et al., 2007 | ||
| Genetic reagent (D. melanogaster) | w; squpp32/CyO; TM3 Sb/TM6 Tb | Pane et al., 2007 | ||
| Cell line (D. melanogaster) | S2 | DRSC | FLYB:FBtc0000181; RRID:CVCL_Z992 | Cell line maintained in T. Kai lab |
| Antibody | Anti-Squ (rat polyclonal) | This study | IF (1:5000) WB (1:1000) |
|
| Antibody | Anti-Spn-E (rat polyclonal) | Lin et al., 2023 | WB (1:500) | |
| Antibody | Anti-Ago3 (rat polyclonal) | Lin et al., 2023 | WB (1:200) | |
| Antibody | Anti-Aub (guinea pig polyclonal) | Lim et al., 2022 | WB (1:1000) | |
| Antibody | Anti-Piwi (mouse monoclonal G-1) | Santa Cruz | Cat# sc-390946 | WB (1:1000) |
| Antibody | Anti-α-Tubulin (mouse monoclonal DM1A) | Santa Cruz | Cat# sc-32293; RRID:AB_628412 | WB (1:1000) |
| Antibody | Anti-guinea pig HRP-conjugated (rabbit polyclonal) | Dako | Cat # P0141; RRID:AB_628412 | WB (1:1000) |
| Antibody | Anti-rat HRP-conjugated (rabbit polyclonal) | Dako | Cat # P0450; RRID:AB_2630354 | WB (1:1000) |
| Antibody | Anti-mouse HRP-conjugated (goat polyclonal) | Bio-Rad | Cat # 1706516; RRID:AB_2921252 | WB (1:3000) |
| Antibody | Anti-rabbit HRP-conjugated (goat polyclonal) | Bio-Rad | Cat # 1706515; RRID:AB_11125142 | WB (1:3000) |
| Antibody | Anti-DDDDK-tag HRP-conjugated (mouse monoclonal) | MBL | Cat# M185-7; RRID:AB_2687989 | WB (1:1000) |
| Antibody | Anti-Myc-tag HRP-conjugated (mouse monoclonal) | MBL | Cat# M192-7; RRID:AB_3678890 | WB (1:1000) |
| Recombinant DNA reagent | Spn-E (plasmid) | Lin et al., 2023 | Myc-tag mK2-tag | |
| Recombinant DNA reagent | Aub (plasmid) | Patil and Kai, 2010 | Myc-tag | |
| Recombinant DNA reagent | BoYb (plasmid) | This study | Myc-tag Flag-tag |
|
| Recombinant DNA reagent | Me31B (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Vret (plasmid) | This study | Flag-tag | |
| Recombinant DNA reagent | Shu (plasmid) | This study | Flag-tag | |
| Recombinant DNA reagent | SquWT (plasmid) | This study | Flag-tag GFP tag |
|
| Recombinant DNA reagent | Squ4A (plasmid) | This study | Flag-tag GFP tag |
|
| Recombinant DNA reagent | SquE107A (plasmid) | This study | Flag-tag GFP tag |
|
| Recombinant DNA reagent | SquE109A (plasmid) | This study | Flag-tag GFP tag |
|
| Recombinant DNA reagent | SquR115A (plasmid) | This study | Flag-tag GFP tag |
|
| Recombinant DNA reagent | SquK163A (plasmid) | This study | Flag-tag GFP tag |
|
| Recombinant DNA reagent | Tej (plasmid) | Patil and Kai, 2010 | Flag-tag | |
| Recombinant DNA reagent | Vas (plasmid) | Patil and Kai, 2010 | Flag-tag | |
| Recombinant DNA reagent | Mei-W68 (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | CSN3 (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Pka-C1 (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Vps25 (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Nup44A (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Nclb (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Spn-D (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Jagn (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Rab7 (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Baf (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Mats (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Abo (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Rab11 (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Vls (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Hsc70-4 (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Piwi (plasmid) | This study | Flag-tag | |
| Recombinant DNA reagent | Twf (plasmid) | This study | Myc-tag | |
| Recombinant DNA reagent | Brn (plasmid) | This study | Myc-tag | |
| Commercial assay or kit | anti-FLAG magnetic beads | MBL | Cat# M185-11R | |
| Commercial assay or kit | anti-Myc magnetic beads | Thermo Fisher | Cat# 88842 | |
| Commercial assay or kit | Dynabeads protein A | Thermo Fisher | Cat# 10001D | |
| Commercial assay or kit | Dynabeads protein G | Thermo Fisher | Cat# 10003D | |
| Chemical compound, drug | Hilymax | Dojindo | Cat# 342-91103 | Transfection in S2 |
| Chemical compound, drug | Signal Enhancer HIKARI | Nacalai Tesque | Cat# 02270-81 | Western blotting |
| Chemical compound, drug | Chemi-Lumi One reagent kit | Nacalai Tesque | Cat# 07880-54 | Western blotting |
| Chemical compound, drug | Fluoro-Keeper Antifade Reagent | Nacalai Tesque | Cat# 12593-64 | |
| Software, algorithm | AlphaFold v2.2 | Developed by DeepMind | RRID:SCR_025454 | Installed in SQUID (Osaka University) |
| Software, algorithm | ImageJ | Schneider et al., 2012 |