Table 2.
Antibodies used in the study.
| Epitope | Control tissue | Supplier/product number | Working concentration | Epitope retrieval | Host/clonality | Secondary antibody | Supplier/product number | Detection system |
|---|---|---|---|---|---|---|---|---|
| Cleaved Caspase-3 | Tonsil | R&D systems AF835 | 0.2 μg/mL | Citrate | Rabbit/polyclonal | Goat-anti-rabbit | Vector Laboratories BA-1000 | ABC |
| CD80 | Tonsil | Bioss Bs-10340R | 1.25 μg/mL | Citrate | Rabbit/polyclonal | Goat-anti-rabbit | Vector Laboratories BA-1000 | ABC |
| CD86 | Tonsil | Antibodies-online ABIN736701 | 2.5 μg/mL | Citrate | Rabbit/polyclonal | Goat-anti-rabbit | Vector Laboratories BA-1000 | ABC |
| CD204 | Histiocytic sarcoma | Trans genic Inc. SRA-E5 | 0.5 μg/mL | Citrate | Mouse/monoclonal | Goat-anti-mouse | Vector Laboratories BA-9200 | ABC |
| Iba-1 | Tonsil | Novus bio NBP2-19019 | 0.06 μg/mL | Citrate | Rabbit/polyclonal | Goat-anti-rabbit | Vector Laboratories BA-1000 | ABC |
| Ki-67 | Small intestine | Dako MIB-1 M7240 | 0.92 μg/mL | Citrate | Mouse/monoclonal | Goat-anti-mouse | Vector Laboratories BA-9200 | ABC |
| Mx1 | Brain (canine distemper infected) | M143 (provided by Prof. Kochs, Institute of Virology, Freiburg University) | 0.6 μg/mL | Citrate | Mouse/monoclonal | Goat-anti-mouse | Vector Laboratories BA-9200 | ABC |
| PD-L1 | Tonsil | Abcam 233482 | 0.5 μg/mL | EDTA | Rabbit/polyclonal | Goat-anti-rabbit | Vector Laboratories BA-1000 | ABC |
| STING | Bronchus | Novus bio NBP2-24683 | 5 μg/mL | Citrate | Rabbit/polyclonal | Goat-anti-rabbit | Vector Laboratories BA-1000 | ABC |
| Survivin | Squamous cell carcinoma | Novus bio NB500-201 | 5 μg/mL | Citrate | Rabbit/polyclonal | Goat-anti-rabbit | Vector Laboratories BA-1000 | ABC |
CD, cluster of differentiation; Iba-1, ionized calcium-binding adapter molecule 1; PD-L1, programmed death ligand 1; EDTA, ethylenediaminetetraacetic acid; STING, stimulator of interferon genes; ABC, avidin-biotin-complex.