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. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: J Biol Chem. 2005 May 16;280(29):26974–26983. doi: 10.1074/jbc.M504453200

Fig. 3. Electrophoretic analysis of total cell extracts expressing wild-type and mutant myosins, the purified myosins and the myosin light chains after phosphorylation of the regulatory light chains.

Fig. 3

A, SDS-PAGE of equivalent amounts of null cells expressing either Wt myosin II heavy chain and each of the 22 mutant heavy chains. B, SDS-PAGE of purified myosins used for biochemical assays before phosphorylation of the regulatory light chains. The positions of the heavy chain (HC) and regulatory (RLC) and essential (ELC) light chains are shown. C, urea-glycerol PAGE of the purified myosins after phosphorylation of the regulatory light chains. Note that almost all of the minor impurities were removed when the myosins were polymerized after phosphorylation. The first lane is unphosphorylated Wt. Positions of the unphosphorylated regulatory light chain (RLC), phosphorylated regulatory light chain (P-RLC) and essential light chains (ELC) are shown.