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. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: J Biol Chem. 2005 May 16;280(29):26974–26983. doi: 10.1074/jbc.M504453200

Fig. 9. S1 binding to F-actin.

Fig. 9

Kobs for the quenching of fluorescence of pyrene-labeled actin added to 0.25 μM S1 in the absence of nucleotide. The slopes of the lines are the second order rate constants, k−6 (Table II). At 0.5 μM F-actin, the actin/S1 concentration ration is too low for pseudo-first order kinetics and, therefore, the values for kobs are approximations but they are consistent with the rest of the data.