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. 2025 Apr 9;16:1557583. doi: 10.3389/fimmu.2025.1557583

Figure 11.

Figure 11

Systemic clodronate liposome delivery reduces CIA+LPS co-exposure induced lung tissue inflammation, collagen deposition, infiltrating CCR2+ monocyte-macrophage cells and myeloperoxidase (MPO)+ neutrophils, vimentin expression, and lung CIT and MAA autoantigen expression. (A) Representative images from treatment groups stained by H&E, collagen by trichrome, CCR2 (red), and MPO (red), vimentin (teal), citrulline (CIT, green), and malondialdehyde-acetaldehyde (MAA, red) modified proteins with DAPI nuclei staining (blue) by confocal microscopy. Scatter plots with bars depict mean with SD of (B) semi-quantitative lung inflammatory score and integrated density of (C) Collagen by trichrome, (D) CCR2 and (E) MPO, (F) vimentin, (G) CIT, and (H) MAA quantified per each mouse. Line scale denotes 100 μm. n=8 mice/group. Symbols represent statistical significance versus vehicle liposome+saline exposures (#) or between CIA+LPS mice treated with vehicle versus clodronate liposomes (*). ####<0.0001; ###,***0.0001 to 0.001; **0.001 to 0.1, *0.01 to 0.05.