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. 2025 Apr 9;16:1557583. doi: 10.3389/fimmu.2025.1557583

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Copyright © 2025 Poole, Schwab, Thiele, Wyatt, Nelson, Schanze, Gleason, Duryee, England and Mikuls

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Clustering by tSNE algorithm of lung monocytes-macrophages from representative of each Sham, LPS, CIA, and CIA+LPS treatment group. (A) tSNE mapping of the data applied to non-debris, single, live CD45⁺Ly6G^-lymphocyte gate^- cells (30,000 cells/sample) of a representative mouse lung combined and from each group (sham, LPA, CIA, CIA+LPS). (B) Multigraph overlays of staining intensity (blue: low and red: high) of cell surface markers utilized for delineating lung monocyte-macrophage subpopulations demonstrated by contour plots.