Fig. 4. Relative expression of luciferase in COS cells cotransformed by constructs containing CRE, CaRe, AP1, NF-κB and SRE elements and different CRFR1 isoforms.
(A) Stimulation by CRF.(B) Stimulation by CRF, forskolin and PMA (TPA) and inhibition by PKA inhibitor (H89). Significant differences between controls and CRF-stimulated cells (P < 0.05) are denoted with an asterisk (*). Open circles denote significant differences between CRF-stimulated cells (pCRFR1a + empty vector and pCRFR1a + pCRFR1g) (P < 0.05).