Table 1. Primer sequences.
Gene | Primers | Primer sequences | Primer location | Amplified band (bp) |
---|---|---|---|---|
Human genes | ||||
FDX1 | P553 | GTGATTCTCTGCTAGATGTTG | Exon 2 | 257 |
P554 | GGCACTCGAACAGTCATATTG | Exon 4 | ||
FDXR | P557 | ATTAAGGAGCTTCGGGAGATG | Exon 7 | 380 |
P558 | CTCTTATACCCAATGCTGCTG | Exon 10 | ||
CYP11A | First pair | |||
P561 | GCCTTTGAGTCCATCACTAAC | Exon 4 | 628 | |
P562 | CCAGTGTCTTGGCAGGAATC | Exon 8 | ||
Nested pair | ||||
P563 | ATGTGGCTGCATGGGACGTG | Exon 4 | 390 | |
P564 | TCTGCAGGGTCACGGAGATG | Exon 7 | ||
Mouse genes | ||||
FDX1 | P581 | AAATTGGCGACTCTCTGCTAG | Exon 2 | 295 |
P582 | CTTGCTCATGTCAACAGACTG | Exon 4 | ||
FDXR | P583 | CTTGGAGTCATCCCCAACAC | Exon 10 | 281 |
P584 | TGGCCTCGAGAGACTTCCTC | Exon 12 | ||
CYP11A | P585 | AGACTTCTTTCGACTCCTCAG | Exon 4 | 693 |
P586 | CTGAAGTTCTCCAGCAGATTG | Exon 8 |