FIGURE 2.

Effects of increasing substrate delivery on mitochondrial energetics. AcCoA concentration is varied and the steady-state behavior of the model analyzed according to a continuation analysis (see text). The correlative changes in the steady-state behavior of V_O2 and NADH versus (A) AcCoA and (B) ΔΨ_m are depicted when AcCoA is systematically changed. The simulation was run with the following set of parameters: V_maxANT = 5.0 mM s⁻¹; ΔpH = −0.6; ρ^res = 0.048 mM; ADPi = 0.05 mM; mM s⁻¹; ρ^F1 = 0.06 mM; [Ca²⁺]_i = 0.5 μM; g_H = 0.01 mM s⁻¹ V⁻¹. The rest of the parameters are as indicated in Table 3. (C) Simulation results match experimental data obtained with pig heart mitochondria, redrawn from Territo et al. (2000).