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. 2005 Jun 29;25(26):6156–6166. doi: 10.1523/JNEUROSCI.1017-05.2005

Figure 6.

Figure 6.

Role of the BDNF prodomain on activity-dependent BDNF secretion. A, Lentiviral constructs containing differential regions of the BDNF prodomain (Box1, Box2, Box3, Box2+ 3) fused to GFP or GFP alone were expressed in PC12 cells transfected with N-terminal HA-BDNF. After 48 h, media were collected under depolarization secretion conditions, as described in Materials and Methods, and analyzed by ELISA. Results are presented as a mean ± SEM determined from six independent experiments (**p < 0.001; Student's t test). B, Differentiated PC12 cells were transfected with mutant forms of BDNF lacking certain regions of the prodomain (ΔBox1, ΔBox2′, ΔBox3, ΔBox2 + 3). After 48 h, media were collected under depolarization secretion conditions, as described in Materials and Methods, and analyzed by ELISA. Results are presented as a mean ± SEM determined from analysis of six independent experiments (**p < 0.001; Student's t test). WT, Wild type.