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. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: Development. 2004 Nov 3;131(23):5935–5945. doi: 10.1242/dev.01465

Fig. 4.

Fig. 4

Slit is expressed in the developing optic lobe. (A,B) Third instar nervous system stained with anti-Slit. (A) Slit is expressed in the medulla neuropil and the base of the lamina (arrow), as well as the ventral ganglion midline and mushroom bodies. (B) In slitdui mutants, Slit expression is greatly reduced in the optic lobe and ventral ganglion, although robust mushroom body staining is still observed. (C) Slit mRNA is expressed by cells surrounding the medulla. (D,E) Third instar visual systems stained with anti-Slit (magenta) and anti-Repo (green). (D) Slit protein is found throughout the medulla neuropil (arrow), which is surrounded by medulla neuropil glia. (E) Slit is present in the lamina neuropil and surrounds the epithelial, marginal and medulla glia. (F) Similar view as in E, stained with anti-Slit (magenta) and Sca:GFP (green). (G) Horizontal view stained with anti-Slit (red), anti-Elav (blue) and Sca:GFP (green). Slit protein localizes immediately adjacent to distal cell neurons at the base of the lamina and the optic chiasm. (H) Summary of Slit expression. (I) Third instar eya2 mutant visual system stained with anti-Slit. Slit protein is expressed in the medulla neuropil (arrow) in the absence of photoreceptor innervation. (I,J) Expression of the slitl(2)k05248 (Slit:lacZ) enhancer trap. (J,K) Optic lobes stained with anti-lacZ (red) and anti-Repo (blue). (J) Slit:lacZ is expressed in medulla glia (arrowhead) and cells in the medulla cortex (arrow). (K) Slit:lacZ cells in medulla cortex (arrow) lie adjacent to medulla neuropil glia. (L) Optic lobe stained with anti-lacZ (upper panel), anti-Elav (middle panel) and a merged image (lower panel) with anti-lacZ in magenta and anti-Elav in green. Slit:lacZ cells in medulla cortex (arrows) coexpress varying levels of neuronal marker Elav. ep, epithelial glia; lamc, lamina cortex; lamn, lamina neuropil; lobc, lobula cortex; ma, marginal glia; mb, mushroom bodies; medc, medulla cortex; medn, medulla neuropil; mg, medulla glia; mng, medulla neuropil glia; vg, ventral ganglion midline.