Fig. 7. Exogenous (Exo) APH-1, PEN-2 and/or PS1 expression partially restore endogenous (Endo) PEN-2 level in Nct KO cells.
APH-1, PEN-2, or PS1 expression constructs was transiently transfected into Nct KO cells individually or collectively. Antibody PNT2 was used to detect both endogenous PEN-2 and exogenous hemagglutinin-PEN-2. α-Tubulin was used as control.