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. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: Immunol Invest. 2004 May;33(2):213–233. doi: 10.1081/imm-120034231

Figure 6A. Diagram of the IL-6 gene structure and GFP-IL-6 3′UTR Reporter Construction.

Figure 6A

Relevant cis elements (5′UTR, coding region and 3′UTR) discussed in proposal are indicated. As part of the preliminary data presented in this section, the murine IL-6 3′UTR (~390 bps) has been cloned into pEGFP-C1 vector where the SV40 poly adenylation site has been removed permitting expression of the EGFP gene under control of the CMV promoter. Stable clones of expressing this construct have been obtained in MC3T3-E1, NIH 3T3, and MC3T3-E1cells immortalized with SV40 large T antigen (designated MCT).