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. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: Immunol Invest. 2004 May;33(2):213–233. doi: 10.1081/imm-120034231

Figure 6d. p38 MAP kinase is required for IL-1β-induced eGFP gene expression in MC6 cells.

Figure 6d

MC6 cells were treated with SB203580 (2 or 5μM), then stimulated with IL-1β (1ng/ml) for 18 hours. Total RNA was isolated and real time RT-PCR performed for eGFP and GAPDH using SybrGreen dye (ABI) on an iCycler PCR machine (BioRad). Threshold cycle values were used to quantitate normalized expression through Q-Gene Software and expressed as ratio of target gene to reference gene (eGFP: GAPDH) compared to control untreated cells.