Skip to main content
. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: J Biol Chem. 2005 May 20;280(28):26278–26286. doi: 10.1074/jbc.M414467200

Figure 3.

Figure 3

Plasma membrane association of GRP78, NCK, and PAK-2 in 1-LN cells stimulated with α2M*. See EXPERIMENTAL PROCEDURES section for details. Panel A: GRP78 in the GRP78 immunoprecipitate from plasma membrane. Panel B: PAK-2 in the GRP78 immunoprecipitate from plasma membrane. Panel C: NCK in GRP78 immunoprecipitate from plasma membrane. The lanes are: (1) buffer and (2) α2M* (50 pM/10 min). Panel D amd J: Effect of silencing the expression of the GRP78 gene on association of PAK-2. Panel E and K: Effect of silencing the expression of the GRP78 gene on the association of NCK. Panel F: Protein loading control, actin. Panel G and I: Effect of silencing the expression of the GRP78 gene on GRP78 protein levels, and Panel H protein loading control GADPH. The lanes in Panel D, E, F, and G are: (1) buffer; (2) α2M*; (3) dsRNA GRP78 then α2M*; and (4) scrambled dsRNA then α2M*. The lanes in Panel I, J, and K are: (1) buffer; (2) α2M*; (3) dsRNA GRP78 and (4) dsRNA GRP78 then α2M*. The immunoblots shown are representative of three to four independent experiments.