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. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: Mol Microbiol. 2005 Jun;56(6):1416–1429. doi: 10.1111/j.1365-2958.2005.04638.x

Fig. 5.

Fig. 5

Norfloxacin treatment causes formation of shortened simple-Y molecules. DNA was isolated from JH39 cells carrying pBR322 and digested with EcoRI. The samples were as follows:

A. DNA was isolated under cleavage conditions (CLV) from untreated cells (UTR).

B. DNA was isolated under cleavage conditions from norfloxacin-treated cells (NOR).

C. DNA was isolated under resealing conditions (RSL) from norfloxacin-treated cells.

The illustrations below indicate the various arcs seen after EcoRI digestion, where B, bubble; LM, linear monomer; SSY, shortened simple-Y; Y, simple-Y; DY, double-Y; AY, asymmetric-Y; and OC, open circular monomer.