Skip to main content
. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: Mol Microbiol. 2005 Jun;56(6):1416–1429. doi: 10.1111/j.1365-2958.2005.04638.x

Fig. 6.

Fig. 6

Norfloxacin treatment causes formation of a sigma arc in undigested DNA. pBR322 DNA was isolated from JH39 under the following conditions:

A. Cleavage conditions (CLV) from untreated cells (UTR).

B. Cleavage conditions from norfloxacin-treated cells (NOR).

C. Resealing conditions (RSL) from norfloxacin-treated cells.

None of the samples were treated with restriction enzymes.

The illustrations indicate the various arcs, where B, bubble (blue); LM, linear monomer; LD, linear dimer; Y, simple-Y (green); TTC, two-tailed circle; S, sigma (red); OC, open circular monomer; CCC, covalently closed circle; CCC RI, covalently closed circle replication intermediate; TopCCC, topoisomers of CCCs; CCC2, dimer of CCC; and CCC/OC, CCC in first dimension, OC in second dimension.