Table 2.
Comparison of array results and RT-PCR results for selected genes. Gene expression percentages are reported relative to the control values. F/ WR: Fasting Weight Reduction.
Genes | Assay | High Fat vs. Control | F/ WR vs. Control |
IL6st | Array | 154 ± 21%* | 144 ± 21%*† |
RT-PCR | 167 ± 19%* | 185 ± 15%*† | |
PTP4a2 | Array | 71 ± 4%* | 89 ± 3%* |
RT-PCR | 75 ± 16% | 94 ± 18% | |
RGS3 | Array | 35 ± 5%* | 54 ± 8%* |
RT-PCR | 38 ± 9%* | 59 ± 8%* | |
G6P | RT-PCR | 476 ± 72% | 769 ± 216%* |
PCK1 | RT-PCR | 132 ± 28% | 217 ± 80%* |
Malic Enzyme | RT-PCR | 9.1 ± 1.5%* | 0.1 ± 0.1%* |
*Indicates that the measurements were significantly different from control values at P < 0.01.
†Indicates that the measurements made on the micro array were significantly different from the RT-PCR measurement at P < 0.05.