Fig. 2.
Activation of the JNK-signaling pathway by expression of hepact in scrib–/–, in rafact, and in scrib–/–, rafact clones causes elimination of mutant tissue from the adult eyes. Eyes carrying mutant clones of the indicated genotypes are shown in normal illumination (Left image of each panel) or by using epifluorescence to visualize GFP marked clonal areas (Center image). The image on the Right of each panel illustrates the growth of GFP-labeled clonal tissue of the respective genotypes during larval development. Eye imaginal discs of the scrib–/–, rafact larvae fuse with the brain and cannot be distinguished from other organs. Eighty percent of the scrib–/–, rafact animals cannot pupate and die as giant larvae. Note that the tissue of scrib–/– tumors (C) and of the Rafact-expressing tumors (E) are all labeled by GFP, whereas the overgrowth in animals in which rafact, hepact (F) or rafact, scrib–/–, hepact (H) clones had been induced is GFP-negative and thus is not clonal in origin.