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. 2005 Sep 6;102(37):13230–13235. doi: 10.1073/pnas.0505070102

Fig. 2.

Fig. 2.

Effect of DST, anti-CD45RB, and anti-CD154 treatment on alloreactive T and B cells. (A) Frequency of IFN-γ-producing donor-specific T cells in C57BL/6 recipients 10 d after transplantation with BALB/c skin. Recipients were treated as indicated. Recipient splenocytes (106) were incubated with irradiated donor splenocytes, and frequency of IFN-γ-producing cells was determined by enzyme-linked immunospot. Data are expressed as mean ± SEM of triplicate wells and are representative of four independent experiments. The frequency after DST plus anti-CD45RB plus anti-CD154 treatment was consistently lower than other groups (P < 0.0001). (B) Circulating alloantibody in C57BL/6 recipients after BALB/c skin transplantation. Naïve BALB/c splenocytes were incubated with 50 μl of serially diluted sera from recipient mice on d 10. Cells were then incubated with anti-mouse IgG1 fluorochrome conjugate vs. an isotype-matched control antibody. The median fluorescence of each sample was compared by flow cytometry. Data are means ± SEM from three mice. *, P < 0.05 vs. anti-CD45RB plus anti-CD154 with or without DST.