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. 2005 Sep 1;102(37):13236–13241. doi: 10.1073/pnas.0506218102

Fig. 5.

Fig. 5.

Knockdown of Pu.1 expression in CD34+ cells induces both Ebf and Pax-5 expression. (Upper) Ebf expression appears in Pu.1 siRNA-treated CD34+ EB cells primed by mGM-CSF and mIL-3 treatment for 72 h. Lane 1, untreated cells; lane 2, cells treated with control Lamin A siRNA; lane 3, cells treated with Pu.1 siRNA. Representative data from one of three individual experiments are shown. (Lower) Pax-5 expression is increased in Pu.1 siRNA-treated CD34+ EB cells. Lane 1, untreated cells; lane 2, cells treated with control Lamin A siRNA; lane 3, cells treated with Pu.1 siRNA. Representative data from one of three individual experiments are shown.