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. 2005 Sep 1;102(37):13236–13241. doi: 10.1073/pnas.0506218102

Fig. 6.

Fig. 6.

Pro-B cell can be further differentiated to mature B cells. (A) Frequency of functional B cell (CD19+IgM+) formation from CD19+CD43+CD45R+ pro-B cells. (B) CD21 expression analysis by flow cytometry. Cytokine stimulation (mIL-7 and mIL-10) is able to up-regulate CD21 expression in these B cells. Representative data from one of three individual experiments are shown. (C) sIgM assay by ELISA on CD19+CD43+CD45R+ cells stimulated by LPS. Data are the mean ± SD of three independent experiments. Lane 1, untreated cells; lane 2, cells treated with LPS. (D) LPS up-regulates CD80 expression in the CD19+CD43+CD45R+ cell population (*, P < 0.01).