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. 2005 Sep 1;102(37):13236–13241. doi: 10.1073/pnas.0506218102

Fig. 7.

Fig. 7.

Knockdown of Pu.1 expression results in mIL-7R expression on CD34+ EB cells cultured either in maintenance conditions (mSCF alone) or myeloid differentiation conditions (mGM-CSF and mIL-3). (Upper) CD34+ EB cells cultured with mSCF did not express mIL-7R when treated with control siRNA; however, upon treatment with Pu.1 siRNA, mIL-7R expression was induced and detected by flow cytometry. Representative data of one of three individual experiments are shown. (Lower) In CD34+ EB cells cultured with mGM-CSF and mIL-3, mIL-7R expression was also induced when cells were treated by Pu.1 siRNA, but not control siRNA. Representative data from one of three individual experiments are shown.