Fig. 8.

Knockdown of Pu.1 expression on CD34⁺ EB cells enhances the frequency of CD19⁺CD43⁺CD45R⁺ cell production mediated by mIL-7, mIL-11, and mFL in vitro. CD34⁺ EB cells were transfected with either Lamin A siRNA (control) or Pu.1 siRNA (50 nM), and cultured with or without OP9 cells and with or without cytokines mIL-7, mIL-11, and mFL for 72 h. Cells were then collected and analyzed by flow cytometry for cell surface marker expression. Data are the mean ± SD of three independent experiments. Pu.1 siRNA-treated CD34⁺ cells showed a 10-fold increase in the production of CD19⁺CD43⁺CD45R⁺ cells compared with the cells treated with control siRNA (*, P < 0.01).