Table 3. Kinetic parameters for basal and assembly-stimulated GTPase activity of wild-type and mutant dynamins measured at 37°C.

	Basal GTPase activity		Lipid tubule-stimulated GTPase activity
Dynamin	kcat, min-1	KM, μM GTP	kcat, min-1	KM, μM GTP
WT	1.45 ± 0.026	46.0 ± 0.40	123.7 ± 7.9	30.0 ± 10.0
R59K	0.474 ± 0.054	96.0 ± 4.1	25.5 ± 0.97	18.0 ± 5.0
R59A	0.980 ± 0.060	241.0 ± 43.0	69.7 ± 3.6	211.0 ± 33.0

Values are averages ± SD from three independent experiments.